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KMID : 0380020060210020123
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 2 p.123 ~ p.128
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High-Yield Production of Astragalosides from Transgenic Hairy Root Cultures of Astragalus membranaceus
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Hwang Sung-Jin
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Abstract
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A transgenic hairy root clone AG-04 of Astragalus membranaceus was obtained following co-cultivation of leaf explants with Agrobacterium rhizogenes ATCC15834. This clone was examined for its growth and production of cyclolanostane-type saponins, astragalosides I, II, and III, under various culture conditions. Among the five basal media tested, Shenk and Hildebrandt (SH)(18) medium was best for roots growth and astragalosides production. The maximum root biomass was obtained at inoculum size of 500 mg FRW per flask, initial sucrose concentration of 3%, and shaking speeds of 90 rpm. The astagalosides production was promoted when the hairy root clone AG-04 was cultured at shaking speeds of 120 rpm and light irradiation of 18 h. Astragaloside contents was also stimulated with high initial sucrose concentration ,and the maximum astargalosides contents of 6.21%/g DRW was obtained at initial sucrose concentration of 6%. The addition of chitosan (100 mg/L) to the culture medium was significantly increased astragalosides production. This was 2.1 times higher than that obtained in a control culture without chitosan.
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KEYWORD
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Astragalus membranaceus, hairy root, astragalosides, inoculum size, Light irradiation shaking speeds, sucrose concentration, chitosan
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